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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/10/2023 |
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Actualizado : |
24/10/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PÉREZ-RUCHEL, A.; BRITOS, A.; ALVARADO, A.; FERNÁNDEZ-CIGANDA, S.; GADEYNE, F.; BUSTOS, M.; ZUNINO, P.; CAJARVILLE, C. |
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Afiliación : |
ANALÍA PÉREZ-RUCHEL, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; ALEJANDRO BRITOS, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; AMAYRI ALVARADO, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av. Italia 3318, 11600 Montevideo, Uruguay; FREDERIK GADEYNE, Royal Agrifirm Group, Landgoedlaan 20, 7325 AW Apeldoorn, the Netherlands; MARIANA BUSTOS, Royal Agrifirm Group, Landgoedlaan 20, 7325 AW Apeldoorn, the Netherlands; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av. Italia 3318, 11600 Montevideo, Uruguay; CECILIA CAJARVILLE, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay. |
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Título : |
Impact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Animal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763 |
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ISSN : |
0377-8401. |
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DOI : |
10.1016/j.anifeedsci.2023.115763 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; email:anapevet@gmail.com -- |
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Contenido : |
This work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearance was the lowest for TSBM followed by PHY (P < 0.001). The true protein disappearance was lower for TSBM and PHY than for CONTROL and MCFA (P < 0.001). The isovaleric acid proportion was lower for TSBM, MCFA, and PHY than for CONTROL. Treatments TSBM and MCFA registered lower neutral detergent fibre disappearance (P < 0.001) than the other diets but without changes in the quantified fibrolytic genera in the ruminal microbiota. The PHY treatment increased the relative abundance of Succinivibrio spp. and reduced that of Methanobrevibacter relative to that of CONTROL and TSBM, which could be linked to lower methanogenesis. The MCFA treatment increased Succinivibrio compared with TSBM and Succiniclasticum spp. compared with CONTROL. These results indicate that PHY inclusion at 10 g/kg DM decreased rumen protein degradation, similar to TSBM; while MCFA decreased rumen protein degradation to a lesser extent. Furthermore, both additives generated changes in the ruminal microbiota that would be associated with lower methane production. © 2023 Elsevier B.V. MenosThis work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearanc... Presentar Todo |
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Palabras claves : |
Gas production; Microbial protein synthesis; Microbiome profile; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Protein degradation. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 04021naa a2200301 a 4500
001 1064355
005 2023-10-24
008 2023 bl uuuu u00u1 u #d
022 $a0377-8401.
024 7 $a10.1016/j.anifeedsci.2023.115763$2DOI
100 1 $aPÉREZ-RUCHEL, A.
245 $aImpact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; email:anapevet@gmail.com --
520 $aThis work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearance was the lowest for TSBM followed by PHY (P < 0.001). The true protein disappearance was lower for TSBM and PHY than for CONTROL and MCFA (P < 0.001). The isovaleric acid proportion was lower for TSBM, MCFA, and PHY than for CONTROL. Treatments TSBM and MCFA registered lower neutral detergent fibre disappearance (P < 0.001) than the other diets but without changes in the quantified fibrolytic genera in the ruminal microbiota. The PHY treatment increased the relative abundance of Succinivibrio spp. and reduced that of Methanobrevibacter relative to that of CONTROL and TSBM, which could be linked to lower methanogenesis. The MCFA treatment increased Succinivibrio compared with TSBM and Succiniclasticum spp. compared with CONTROL. These results indicate that PHY inclusion at 10 g/kg DM decreased rumen protein degradation, similar to TSBM; while MCFA decreased rumen protein degradation to a lesser extent. Furthermore, both additives generated changes in the ruminal microbiota that would be associated with lower methane production. © 2023 Elsevier B.V.
653 $aGas production
653 $aMicrobial protein synthesis
653 $aMicrobiome profile
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aProtein degradation
700 1 $aBRITOS, A.
700 1 $aALVARADO, A.
700 1 $aFERNÁNDEZ-CIGANDA, S.
700 1 $aGADEYNE, F.
700 1 $aBUSTOS, M.
700 1 $aZUNINO, P.
700 1 $aCAJARVILLE, C.
773 $tAnimal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763
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| Registros recuperados : 14 | |
| 3. |  | MAURENTE BERÓN, M.; FERNÁNDEZ-CIGANDA, S.; GIANNITTI, F.; FRAGA, M. Comparison of PCR and qPCR for the detection of Mycobacterium avium subsp. paratuberculosis in feces and milk of dairy cows in Uruguay: a longitudinal study. (ID-P47). [poster-Infectious Diseases] Session: Posters. In: World Buiatrics Congress (WBC), 31 st., Abstract Book, Madrid, Spain, September 4-8, 2022.| Biblioteca(s): INIA Las Brujas. |
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| 6. | | LOZANO, J.; FERNÁNDEZ-CIGANDA, S.; FABIUS, S.; PICCINI, C.; SCORZA, C.; ZUNINO, P. Efecto tipo-antidepresivo inducido por el tratamiento crónico de una cepa del grupo lactobacillus plantarum en ratas y asociación con la microbiota intestinal. 395. (resúmen) Conferencia 21. Mesa Área Biotecnología. Organiza SUM (Sociedad Uruguaya de Microbiología). In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.86. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 7. | | LOZANO, J.; FERNÁNDEZ-CIGANDA, S.; GONZÁLEZ REVELLO, A.; HIRIGOYEN, D.; MARTÍNEZ, M.; SCORZA, C.; ZUNINO, P. Probiotic potential of GABA-producing lactobacilli isolated from Uruguayan artisanal cheese starter cultures. Journal of Microbiology, 2022, volume 133, issue 3, pages 1610-1619. doi: https://doi.org/10.1111/jam.15664 Article history: Received 18 March 2022; Revised 5 May 2022; Accepted 6 June 2022; Published online September 2022. -- Corresponding author: Zunino, P.; Department of Microbiology, Instituto de Investigaciones Biológicas Clemente Estable,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 8. | | FEDERICI, M.; RIGAMONTI, N.; FERRARI, G.; ROVIRA, P.J.; TORRES, P.; GUERRA, S.; SIMÓN, C.; FERNÁNDEZ-CIGANDA, S. Análisis de comunidades de cianobacterias y microorganismos asociados al sedimento mediante secuenciación del gen 16S ARNr y qPCR en el embalse rincón del bonete. 161. (resúmen) Áreas temáticas: Ciencias del Mar y Limnología. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.145. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 9. | | SILVEIRA, C.S.; GRILLI, M.J.; BERON, M.; CAFFARENA, D.; FRAGA, M.; GIANNITTI, F.; FERNÁNDEZ-CIGANDA, S. Detecção e quantificação do vírus da leucose bovina em terneiros neonatos de estabelecimentos leiteiros do Uruguai. [resumo] In: Encontro Nacional de Diagnóstico Veterinário (ENDIVET), 11, Resumos, Cuiabá, Mato Grosso, Brazil, Novembro 21-23, 2022.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 10. | | SLIMOVICH, L.; CRESCIONINI, A.; SALADA, S.; SILVEIRA, C.S.; FERNÁNDEZ-CIGANDA, S.; DHUNGYEL, O.; FIERRO, S.; FRAGA, M. Determinación de serogrupos circulantes de Dichelobacter nodosusen majadas con diagnóstico clínico de Foot-Rot en ovinos de Uruguay. Resultados Preliminares. [abstract]. In: AUPA, Proceedings del VII Congreso Uruguayo de Producción Animal. Sección Una Sola Salud (Single Health Section), 14 y 15 diciembre 2021. Archivos Latinoamericanos de Producción Animal, 29(Supl.1), p.99-102. (Archivos Latinoamericanos de Producción Animal, Vol.29, Supl.1) Corresponding author: L. Slimovich, Plataforma de Investigación en Salud Animal, INIA-La Estanzuela, mailto:ludmilaslimovich@gmail.com| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 11. | | FABIUS, S.; URBANAVICIUS, J.; FERNÁNDEZ-CIGANDA, S.; PRIETO, J.P.; LOZANO, J.; PICCINI, C.; ZUNINO, P.; SCORZA, C. Efecto del pretratamiento oral con Lactobacillus SPP. en la sensibilización locomotora inducida por cocaína y su influencia en la microbiota intestinal. [resumen] Conferencia 3. Mesa Sociedad de Neurociencias del Uruguay. Mesa VI. Presentaciones orales seleccionadas de estudiantes. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.38. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 12. | | PÉREZ-RUCHEL, A.; BRITOS, A.; ALVARADO, A.; FERNÁNDEZ-CIGANDA, S.; GADEYNE, F.; BUSTOS, M.; ZUNINO, P.; CAJARVILLE, C. Impact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system. Animal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763 Article history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 13. | | GIANNITTI, F.; DORSCH, M.; FERNÁNDEZ-CIGANDA, S.; RABAZA, A.; VÁZQUEZ, S.; CÉSAR, C.; HURTADO, J.; GREIF, G.; RABENECK, D.; BHATNAGAR, J.; RITTER, J. Canine leproid granuloma caused by a member of the Mycobacterium tuberculosis complex. (Brief Report). Journal of Veterinary Diagnostic Investigation. 2023, Volume 35, Issue 4, pages 439-443. https://doi.org/10.1177/10406387231176816 Article history: First published online May 19, 2023; Issue published July 2023. -- Corresponding author: Federico Giannitti, Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Ruta 50,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 14. | | GIANNITTI, F.; SILVEIRA, C.S.; BULLOCK, H.; BERON, M.; FERNÁNDEZ-CIGANDA, S.; BENÍTEZ-GALEANO, M.J.; RODRÍGUEZ-OSORIO, N.; SILVA-FLANNERY, L.; PERDOMO, T.; CABRERA, A.; PUENTES, R.; COLINA, R.; RITTER, J.M.; CASTELLS, M. Bovine Polyomavirus-1 (Epsilonpolyomavirus bovis): An emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans. Viruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042 Article history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022.
Academic Editors: Fernando Bauermann and Mayara Maggioli.
Correspondence authors: Giannitti, F.; Plataforma de...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 14 | |
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